The contribution represents a brief overview of results of landscape-ecological, as well as human-ecological research conducted during recent period in Slovakia. A special interest is given to the regional (sub-national) dimension. The set of 41 indicators, aggregated to 6 groups pursuing six sustainable development (SD) dimensions has been used with the aim to characterise the state and development of Slovak administrative regions. The next step was the introduction of the Integrated Sustainable Development Index (ISDI) in order to express the global situation of Slovak regions from the SD perspective. Authors also payed attention to regional disparities with the aim to find answers to questions, whether the development of Slovak regions during the second half of the 1990s was compatible with SD principles and criteria, or not, whether the development of the individual SD dimensions was synchronised, and how significant and relevant were disparities among administrative regions in Slovakia.