Long-term fertilization effects were evaluated including NPKCa and N application in the lower altitudes. The research plot Moštice was established on the Forest District Krymlov territory (Forest District Kostelec today) in the stand 638 b1 (today code 13 D11), at the altitude 400 m a.s.l. on a mild NW slope, forest type was determined as Luzulo pilosae Abietum (4O1). The soil (Luvisol) is loamy, with loess enrichment. At the age of sampling, the stand age was 108 years. Fertilizers were used in the period 1965–1976, evaluation of the soil state was done in 1967 (before) and 2002 years. After 25–35 years, only slight effects of fertilization are detectable in the humus forms and complex soil profile – surface humus accumulation, soil chemistry, as well as nutrient contents. Complex fertilization was reflected by higher site fertility in general, the N-fertilization only in the progress of acidification. During the period 1967–2002, a strong general trend of acidification is obvious caused both by the acid deposition and the Norway spruce monoculture-based forestry.